Synthesis and ribonuclease activity of oligonucleotides with N3-terpyridine?Cu(II)-linked thymine residues - Oligonucleotides

Synthesis and ribonuclease activity of oligonucleotides with N3-terpyridine?Cu(II)-linked thymine residues
2006
Kazuhiro Hara, Masaya Kitamura and Hideo Inoue
Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan
Nucleic Acids Symposium Series 2006
In previous studies of RNA cleavage, we prepared 2'-O-methyloligonucleotide?terpyridine?Cu(II) complex(es) as sequence-specific artificial ribonucleases, in which the terpyridine group was attached to the nucleoside-sugar moiety. We found that the antisense 2'-O-methyloligomer with two terpyridine?Cu(II) complexes at contiguous internal sites was most active, and precisely cleaved the target RNA oligomer at the predetermined site. In order to explore more efficient cleavers, we have constructed a structurally similar 18-mer, but with two modified thymidine residues, in which the complex was attached to the base-N3 position by a hydroxyethyl linker. The reaction of the target RNA 24-mer with a 10-fold excess of the new agent, in the presence of Cu(II) ions, and at pH 7.5 and 37?C, revealed that the substrate was almost completely cleaved after 10 hr, and that the cleavage occurred at two contiguous sites. The activity of the new cleaver was more than half of that of the previous cleaver.
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