Synthesis and application of dehydroabietylisothiocyante as a new chiral derivatizing agent for the enantiomeric separation of chiral compounds by capillary electrophoretic
Synthesis and application of dehydroabietylisothiocyante as a new chiral derivatizing agent for the enantiomeric separation of chiral compounds by capillary electrophoretic
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06099734020
Title: Synthesis and application of dehydroabietylisothiocyante as a new chiral derivatizing agent for the enantiomeric separation of chiral compounds by capillary electrophoretic
Authors: Wang, Hengshan; Zhang, Rongcan; Zhao, Shulin; Tang, Lidong; Pan, Yinming
Author affiliation: College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004 Guilin, China
Serial title: Analytica Chimica Acta
Abbreviated serial title: Anal. Chim. Acta
Volume: v 560
Issue: n 1-2
Issue date: Feb 23 2006
Publication year: 2006
Pages: p 64-68
Language: English
ISSN: 0003-2670
CODEN: ACACAM
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: A new chiral derivatizing reagent, dehydroabietylisothiocyante (DHAIC), was synthesized and used for the enantiomeric separation of chiral compounds in capillary electrophoresis (CE). The synthetic route to obtain DHAIC is described. The separation conditions for the chiral separation of several chiral compounds, such as protein amino acids and chiral drug DOPA were optimized. Best results for the chiral separation of DHAIC derivatized amino acids and DOPA were obtained in a running buffer consisted of 50 mM borate (pH 9.5), 5 mM sodium dodecyl sulphate (SDS) and 20% acetonitrile for amino acids and 60 mM Na2HPO4 (pH 8.0), 17 mM SDS and 25% acetonitrile for DOPA. Under the conditions studied, chiral separation of five amino acids including Ser, Val, Ala, Thr, Cys and a chiral drug DOPA as their diastereomeric DHAIC derivatives has been achieved by micellar electrokinetic chromatography (MEKC). ? 2006 Elsevier B.V. All rights reserved.
Number of references: 18
Ei main heading: Cyanides
Ei controlled terms: Synthesis (chemical) - Derivatives - Electrophoresis - Capillarity - Amino acids - Chromatographic analysis
Uncontrolled terms: Enantiomeric separation - Capillary electrophoresis - Derivatization
Ei classification codes: 804.2 Inorganic Compounds - 802.2 Chemical Reactions - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry - 631.1 Fluid Flow, General - 804.1 Organic Compounds
Treatment: Theoretical (THR)
DOI: 10.1016/j.aca.2005.12.041
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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