Surface characterization of human serum albumin and sodium perfluorooctanoate mixed solutions by pendant drop tensiometry and circular dichroism
Surface characterization of human serum albumin and sodium perfluorooctanoate mixed solutions by pendant drop tensiometry and circular dichroism
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 06239917699
Title: Surface characterization of human serum albumin and sodium perfluorooctanoate mixed solutions by pendant drop tensiometry and circular dichroism
Authors: Messina, Paula; Prieto, Gerardo; Dodero, Veronica; Cabrerizo-Vilchez, M.A.; Maldonado-Valderrama, J.; Ruso, Juan M.; Sarmiento, Felix
Author affiliation: Departamento de Fisica Aplicada, Facultad de Fisica, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain
Serial title: Biopolymers
Abbreviated serial title: Biopolymers
Volume: v 82
Issue: n 3
Issue date: Jun 15 2006
Publication year: 2006
Pages: p 261-271
Language: English
ISSN: 0006-3525
CODEN: BIPMAA
Document type: Journal article (JA)
Publisher: John Wiley and Sons Inc., Newark, NJ 07191-8667, United States
Abstract: The interfacial behavior of mixed human serum albumin (HSA)/sodium perfluorooctanoate (C8FONa) solutions is examined by using two experimental techniques, pendant drop tensiometry and circular dichroism spectroscopy. Through the analysis of the surface tension of the mixed solutions, surface competitive adsorption at the air-water interface between C8FONa and HSA is detected. The dynamic adsorption curves exhibit the distinct regimes in their time-dependent surface tension. The nature of these regimes is further analyzed in terms of the variation of the molecules surface areas. As a consequence, a compact and dense structure was formed where protein molecules were interconnected and overlapped. Thus, a reduction of the area occupied per molecule from 100 to 0.2 nm2 is interpreted as a gel-like structure at the surface. The presence of the surfactant seems to favor the formation of this interfacial structure. Finally, measurements of circular dichroism suggests a compaction of the protein due to the association with the surfactant given by an increase of a-helix structure in the complexes as compared to that of pure protein. ? 2006 Wiley Periodicals, Inc.
Number of references: 60
Ei main heading: Proteins
Ei controlled terms: Surface chemistry - Sodium compounds - Spectroscopic analysis - Adsorption - Surface tension - Surface active agents
Uncontrolled terms: Human serum albumin - Sodium perfluorooctanoate - Surface characterization - Pendant drop tensiometry - Circular dichroism
Ei classification codes: 804.1 Organic Compounds - 801.4 Physical Chemistry - 802.3 Chemical Operations - 931.2 Physical Properties of Gases, Liquids & Solids - 803 Chemical Agents and Basic Industrial Chemicals
Treatment: Literature review (LIT); Experimental (EXP)
DOI: 10.1002/bip.20494
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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