Study on the formation and depolymerization of acridine orange dimer in acridine orange?sodium dodecyl benzene sulfonate?protein system

Study on the formation and depolymerization of acridine orange dimer in acridine orange?sodium dodecyl benzene sulfonate?protein system
Received 17 November 2005; accepted 11 January 2006. Available online 3 February 2006.
Fei Wanga, Jinghe Yanga, , , Xia Wua, Xiaobo Wangb, Lishun Fenga, Zhen Jiaa and Changying Guoa
Journal of Colloid and Interface Science
Volume 298, Issue 2 , 15 June 2006
ScienceDirect
aKey Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China
bCenter of Penal Technology, Public Security Department of Guangdong Province, Guangzhou 510050, PR China
Abstract
Experiment indicates that the fluorescence of acridine orange (AO) can be greatly quenched by anionic surfactant sodium dodecyl benzene sulfonate (SDBS), but when protein is added into the AO?SDBS system, the fluorescence intensity of the latter is enhanced. It is considered that SDBS can promote the formation of AO dimer, resulting in the quenching of the fluorescence of AO. When bovine serum albumin (BSA) is added into AO?SDBS system, BSA and SDBS can interact and form negative micelle-like cluster complex with ?aromatic ring stacking,? which destroys the formation conditions of AO dimer and makes some AO dimers turn into monomer, resulting in the fluorescence enhancement of AO?SDBS system. Whereas the positive AO and residual AO dimer are dissolved in the negative BSA?SDBS cluster through electrostatic and hydrophobic forces and form a large association. Here, the fluorescence enhancement of AO?SDBS is considered to originate from the hydrophobic microenvironment provided by BSA and SDBS, the depolymerization of AO dimer and intermolecular energy transfer between BSA and AO.
Graphical abstract
The interaction model of the BSA?AO?SDBS system.
Keywords: Formation; Depolymerization; Acridine orange dimer; Protein; Surfactant

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