Stable composition of immobilized protein having affinity for biotin

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Patent number: US5998155
Publication date: 1999-12-07
Inventor: BURTON STEVEN JAMES (GB); PEARSON JAMES C (GB); EDWARDSON PETER A D (GB)
Applicant: SQUIBB & SONS INC (US)
Classification:
- international: C07D235/02; C12N11/00; C12N11/10; G01N33/569
- european: A61L2/00P2F; A61L24/10F; C07K1/22; C07K14/36; C07K14/465; C07K14/75; C07K17/10; C12P21/06
Application number: US19970904368 19970801
Priority number(s): US19970904368 19970801; US19950556244 19951109
Abstract of US5998155

Compositions and methods for avidin or protein having affinity for biotin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels for binding biotin and may include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the composition during lyophilization and terminal sterilization processes. The compositions have applicability in any instance where avidin agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomers useful for fibrin sealants.
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