Selection of smart aptamers by methods of kinetic capillary electrophoresis

Selection of smart aptamers by methods of kinetic capillary electrophoresis
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06219892133

Title: Selection of smart aptamers by methods of kinetic capillary electrophoresis

Authors: Drabovich, Andrei P.; Berezovski, Maxim; Okhonin, Victor; Krylov, Sergey N.

Author affiliation: Department of Chemistry, York University, Toronto, Ont. M3J 1P3, Canada

Serial title: Analytical Chemistry

Abbreviated serial title: Anal. Chem.

Volume: v 78

Issue: n 9

Issue date: May 1 2006

Publication year: 2006

Pages: p 3171-3178

Language: English

ISSN: 0003-2700


Document type: Journal article (JA)

Publisher: American Chemical Society, Columbus, OH 43210-3337, United States

Abstract: We coin the term "smart aptamers"-aptamers with predefined binding parameters (kon, koff, Kd) of aptamer-target interaction. Aptamers, in general, are oligonucleotides, which are capable of binding target molecules with high affinity and selectivity. They are considered as potential therapeutic targets and also thought to rival antibodies in immunoassay-like analyses. Aptamers are selected from combinatorial libraries of oligonucleotides by affinity methods. Until now, technological limitations have precluded the development of smart aptamers. Here, we report on two kinetic capillary electrophoresis techniques applicable to the selection of smart aptamers. Equilibrium capillary electrophoresis of equilibrium mixtures was used to develop aptamers with predefined equilibrium dissociation constants (Kd), while nonequilibrium capillary electrophoresis of equilibrium mixtures facilitated selection of aptamers with different dissociation rate constants (koff). Selections were made for MutS protein, for which aptamers have never been previously developed. Both theoretical and practical aspects of smart aptamer development are presented, and the advantages of this new type of affinity probes are described. ? 2006 American Chemical Society.

Number of references: 40

Ei main heading: Polymers

Ei controlled terms: Electrophoresis - Capillarity - Reaction kinetics - Cells - Catalyst selectivity - Antibodies

Uncontrolled terms: Aptamers - Capillary electrophoresis - Binding parameters - Oligonucleotides

Ei classification codes: 815.1 Polymeric Materials - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry - 631.1 Fluid Flow, General - 802.2 Chemical Reactions - 461.2 Biological Materials - 803 Chemical Agents and Basic Industrial Chemicals - 804 Chemical Products Generally - 461.9.1 Immunology

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1021/ac060144h

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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