Resonance light scattering technique for the determination of proteins with Congo red and Triton X-100

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Resonance light scattering technique for the determination of proteins with Congo red and Triton X-100
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 8869101

Title: Resonance light scattering technique for the determination of proteins with Congo red and Triton X-100

Authors: Xia Wu1 ; Shuna Sun1 ; Changying Guo1 ; Jinghe Yang1 ; Changxia Sun1 ; Chengrong Zhou1 ; Tao Wu1

Author affiliation: 1 Key Lab. of Colloid & Interface Chem., Shandong Univ., Jinan, China

Serial title: Luminescence. The Journal of Biological and Chemical Luminescence

Abbreviated serial title: Lumin., J. Biol. Chem. Lumin. (UK)

Volume: 21

Issue: 1

Publication date: Jan.-Feb. 2006

Pages: 56-61

Language: English

ISSN: 1522-7235

CODEN: JBCHE7

Document type: Journal article (JA)

Publisher: Wiley

Country of publication: UK

Material Identity Number: H308-2006-002

Abstract: Resonance light scattering (RLS) of Congo red (CR) was greatly enhanced by BSA (HSA) in the presence of Triton X-100 (TX-100). In sodium citrate-HCl buffer (pH 2.7-3.0), the enhanced intensity of resonance light scattering at 360 nm was in proportion to the concentration. The linear relationship was obtained between the resonance light scattering intensity and proteins in the range 5.0?10-8-8.0?10-6 g/mL and 1.0?10-9-6.0?-6 g/mL for BSA and HSA, respectively. Their detection limits were 1.4?-8 g/mL and 2.8?10-10 g/mL (S:N=3), respectively. Synthetic and actual samples were analysed satisfactorily

Number of references: 21

Inspec controlled terms: bio-optics - biochemistry - light scattering - molecular biophysics - proteins

Uncontrolled terms: resonance light scattering - Congo red - Triton X-100 - sodium citrate-HCl buffer - proteins - synthetic samples - actual samples - bovine serum albumin - human serum albumin - 360 nm

Inspec classification codes: A8715M Interactions with radiations at the biomolecular level - A8750B Interactions of biosystems with radiations - A8715D Physical chemistry of biomolecular solutions; condensed states

Numerical data indexing: wavelength 3.6E-07 m

Treatment: Experimental (EXP)

Discipline: Physics (A)

DOI: 10.1002/bio.884

Database: Inspec

Copyright 2006, The Institution of Engineering and Technology
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