Quantitative determination of proteins at nanogram levels by the resonance light-scattering technique with composite nanoparticles of CdS/PAA
Quantitative determination of proteins at nanogram levels by the resonance light-scattering technique with composite nanoparticles of CdS/PAA
Engineering Village 2
2006 Elsevier Inc.
Accession number: 063510095402
Title: Quantitative determination of proteins at nanogram levels by the resonance light-scattering technique with composite nanoparticles of CdS/PAA
Authors: Chen, Hongqi; Xu, Fagong; Hong, Shi; Wang, Lun
Author affiliation: College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu, 241000, China
Serial title: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Abbreviated serial title: Spectrochim. Acta Part A Mol. Biomol. Spectrosc.
Volume: v 65
Issue: n 2
Issue date: October 2006
Publication year: 2006
Pages: p 428-432
Language: English
ISSN: 1386-1425
CODEN: SAMCAS
Document type: Journal article (JA)
Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands
Abstract: This paper describes the development of composite nanoparticles. A novel composite nanoparticle has been prepared by an in situ polymerization method. The nano-CdS has been prepared, then the polymerization of acrylic acid (AA) was carried out by initiator potassium persulfate (KPS) under ultrasonic irradiation. The surface of the composite nanoparticles was covered with abundant carboxylic groups (-COOH). The nanoparticles are water-soluble, stable and biocompatible. Reaction of the composite nanoparticles with proteins results in an enhanced resonance light scattering (RLS) at 380 nm. Based on this, a new resonance light-scattering (RLS) method was developed for the determination of proteins including BSA, HSA and human ?-IgG. Under the optimum conditions, the enhanced RLS intensity is linearly proportional to the concentration of proteins. The liner range is 0.1-15 ?g mL-1 for HSA, 0.2-20 ?g mL-1 for BSA and 0.1-50.0 ?g mL-1 for human ?-IgG, respectively. The method has been applied to the determination of the total protein in human serum samples collected from the hospital and the results are in good agreement with those reported by the hospital. This method proved to be very sensitive, rapid, simple and tolerant of most interfering substances. ? 2005 Elsevier B.V. All rights reserved.
Number of references: 19
Ei main heading: Nanostructured materials
Ei controlled terms: Composite materials - Cadmium sulfide - Proteins - Polymerization - Carboxylic acids - Biocompatibility - Resonance - Light scattering
Uncontrolled terms: Resonance light scattering - Composite nanoparticles - CdS/PAA - Potassium persulfate (KPS)
Ei classification codes: 933.1 Crystalline Solids - 817.1 Plastics Products - 804.2 Inorganic Compounds - 804.1 Organic Compounds - 815.2 Polymerization - 461.9.1 Immunology - 931.1 Mechanics - 741.1 Light/Optics
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1016/j.saa.2005.10.055
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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