Protein and nanoparticle characterisation using light scattering techniques
Protein and nanoparticle characterisation using light scattering techniques
Engineering Village 2
2006 Elsevier Inc.
Accession number: 063710110070
Title: Protein and nanoparticle characterisation using light scattering techniques
Authors: Kaszuba, Michael; Connah, Malcolm T.
Author affiliation: Malvern Instruments Ltd., Enigma Business Park, Malvern WR14 1XZ, United Kingdom
Serial title: Particle and Particle Systems Characterization
Abbreviated serial title: Part. Part. Syst. Charact.
Volume: v 23
Issue: n 2
Issue date: August 2006
Publication year: 2006
Pages: p 193-196
Language: English
ISSN: 0934-0866
CODEN: PPCHEZ
Document type: Journal article (JA)
Publisher: Wiley-VCH Verlag, Weinheim, D-69451, Germany
Abstract: The technique of dynamic light scattering is well suited to the measurement of the size of colloidal dispersions. Traditionally, measurements of large particle sizes or particles that are highly scattering would require high dilution of the sample in order to avoid multiple scattering effects. Conversely, measurement of very small and/ or poorly scattering particles or samples that are very dilute are difficult unless high-powered lasers are used. These problems have been addressed in a light scattering instrument incorporating novel non-invasive backscatter optics (NIBS). This novel optic arrangement maximises the detection of scattered light while maintaining signal quality. In addition to the increased size and concentration range for DLS applications, the increased sensitivity obtained from this optical arrangement also allows for the determination of absolute molecular weight using static light scattering measurements. Accurate molecular weight measurements can be achieved at a single scattering angle for molecules that do not show any angular dependence in their scattering intensity. This paper describes the advantages of using backscatter optics and illustrates the capabilities of the technology by reporting results of measurements from various applications. ? 2006 WILEY-VCH Verlag GmbH and Co. KGaA.
Number of references: 5
Ei main heading: Nanostructured materials
Ei controlled terms: Proteins - Particle size analysis - Light scattering - Backscattering - Molecular weight
Uncontrolled terms: Absolute molecular weight - Dynamic light scattering - Static light scattering - Non-invasive backscatter optics (NIBS)
Ei classification codes: 933.1 Crystalline Solids - 804.1 Organic Compounds - 943.3 Special Purpose Instruments - 741.1 Light/Optics - 711 Electromagnetic Waves - 931.3 Atomic & Molecular Physics
Treatment: Theoretical (THR)
DOI: 10.1002/ppsc.200601030
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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