Protein Purification Protocols - Second Edition

Protein Purification Protocols - Second Edition
Humana Press
Cutler, Paul (SmithKline Beecham Pharmaceuticals, Harlow, UK)
Binding: Hardcover
Pub. Date: December 2003
Pages: 496
ISBN: 1-58829-067-0
Price: $115.00
This new edition of Protein Purification Protocols (1996) completely updates the existing protocols to reflect recent advances and adds the enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins.
1. General Strategies
Doonan, Shawn; Cutler, Paul
pp. 1-14
2. Preparation of Extracts From Animal Tissues
Skehel, J. Mark
pp. 15-20
3. Protein Extraction From Plant Tissues
Fido, Roger J.; Mills, E. N. Clare; Rigby, Neil M.; Shewry, Peter R.
pp. 21-28
4. Extraction of Recombinant Protein From Bacteria
McGettrick, Anne. F.; Worrall, D. Margaret
pp. 29-36
5. Protein Extraction From Fungi
Bridge, Paul D.; Kokubun, Tetsuo; Simmonds, Monique S. J.
pp. 37-46
6. Subecllular Fractionation of Animal Tissues
Ryan, Norma M.
pp. 47-52
7. Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria
Tobin, Alyson K.; Bowsher, Caroline G.
pp. 53-64
8. The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds
Pierpoint, William S.
pp. 65-74
9. Avoidance of Proteolysis in Extracts
Beynon, Robert J.; Oliver, Simon
pp. 75-84
10. Concentration of Extracts
Doonan, Shawn
pp. 85-90
11. Making and Changing Buffers
Doonan, Shawn
pp. 91-100
12. Purification and Concentration by Ultrafiltration
Schratter, Paul
pp. 101-116
13. Bulk Purification by Fractional Precipitation
Doonan, Shawn
pp. 117-124
14. Ion-Exchange Chromatography
Selkirk, Chris
pp. 125-132
15. Hydrophobic Interaction Chromatography
O'Farrell, Paul A.
pp. 133-138
16. Affinity Chromatography
Cutler, Paul
pp. 139-150
17. Dye-Ligand Affinity Chromatography
McGettrick, Anne F.; Worrall, D. Margaret
pp. 151-158
18. Lectin Affinity Chromatography
West, Iris; Goldring, Owen
pp. 159-166
19. Immunoaffinity Chromatography
Cutler, Paul
pp. 167-178
20. Immobilized Metal-Ion Affinity Chromatography
Yip, Tai-Tung; Hutchens, T. William
pp. 179-190
21. Chromatography on Hydroxyapatite
Doonan, Shawn
pp. 191-194
22. Thiophilic Affinity Chromatography and Related Methods
Matejtschuk, Paul
pp. 195-204
23. Affinity Precipitation Methods
Irwin, Jane A.; Tipton, Keith F.
pp. 205-224
24. Isoelectric Focusing
Westermeier, Reiner
pp. 225-232
25. Chromatofocusing
Mantle, Timothy J.; Noone, Patricia
pp. 233-238
26. Size-Exclusion Chromatography
Cutler, Paul
pp. 239-252
27. Fast Protein Liquid Chromatography
Sheehan, David; O'Sullivan, Siobhan
pp. 253-258
28. Reversed-Phase Chromatography of Proteins
Neville, William A.
pp. 259-282
29. Extraction of Membrane Proteins
Ohlendieck, Kay
pp. 283-294
30. Removal of Detergent From Protein Fractions
Ohlendieck, Kay
pp. 295-300
31. Purification of Membrane Proteins
Ohlendieck, Kay
pp. 301-308
32. Lyophilization of Proteins
?'F?g?in, Ciar?n
pp. 309-322
33. Storage of Pure Proteins
?'F?g?in, Ciar?n
pp. 323-338
34. Electroelution of Proteins From Polyacrylamide Gels
Dunn, Michael J.
pp. 339-344
35. Electroblotting of Proteins From Polyacrylamide Gels
Dunn, Michael J.
pp. 345-352
36. Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses
Jones, Neil A.
pp. 353-360
37. Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for
Comprehensive Proteome Analysis Zuo, Xun; Speicher, David W.
pp. 361-376
38. Practical Column Chromatography
Doonan, Shawn
pp. 377-388
39. Detection Methods
Mozdzanowski, Jacek; Burman, Sudhir
pp. 389-410
40. Peptide Proteomics
McNulty, Dean E.; Slemmon, J. Randall
pp. 411-424
41. Multidimensional Liquid Chromatography of Proteins
Watson, Rod; Nadler, Tim
pp. 425-446
42. Mass Spectrometry
Bell, David J.
pp. 447-454
43. Purification of Therapeutic Proteins
Bonnerjea, Julian
pp. 455-462
44. Purification Process Scale-Up
Prince, Karl; Smith, Martin
pp. 463-480