Process for preparing tuckahoe mycelium extracellular polysacchride with anti-tumor activity

/Home/Lyophilization and Protein Formulation Patents/Patent Articles - Lyophilization/European Patents - Lyophilization

Patent Number: CN1587279
Publication date: 2005-03-02
Inventor(s): ZHANG LINA [CN]; JIN YONG [CN]; CHEN LI [CN]
Applicant(s): UNIV WUHAN [CN]
Requested Patent: CN1587279
Application Number: CN200410060650 20040728
Priority Number(s): CN200410060650 20040728
IPC Classification: C08B37/00; C12P19/04; A61K31/715; A61P35/00
Abstract
The present invention relates to the preparation process of tuckahoe mycelium extracellular polysaccharide with antitumor activity. Wild tuckahoe is deep fermented in corn slurry culture medium of pH 5.0-6.0 inside high pressure sterilized fermentation tank, and the culture liquid is collected and spray dried to obtain coarse extracellular polysaccharide. The coarse extracellular polysaccharide is dissolved in distilled water, filtered, H2O2 decolorized, free protein eliminated via Sevag process, clear water dialyzed for 5-7 days, distilled water dialyzed for 3-5 days, decompression distilled and freeze dried to obtain pure extracellular polysaccharide. Intracorporeal active experiment shows that the water soluble heteropolysaccharide has obvious growth inhibiting effect on Sarcoma 180 implanted inside mouse body and no toxic side effect. Extracorporeal active experiment proves its obvious inhibiting effect on the proliferation of HL-60 and has no damage to health monkey kidney cell.
Data supplied from the esp@cenet database - l2