PARTICLE FOR ENZYMATIC ACTIVITY DETECTION AND, UTILIZING THE SAME, METHOD OF ENZYMATIC ACTIVITY DETECTION AND ENZYMATIC ACTIVITY DETECTION TOOL

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PARTICLE FOR ENZYMATIC ACTIVITY DETECTION AND, UTILIZING THE SAME, METHOD OF ENZYMATIC ACTIVITY DETECTION AND ENZYMATIC ACTIVITY DETECTION TOOL
Publication Number: WO/2006/075429 International Application No.: PCT/JP2005/019625
Publication Date: 20.07.2006 International Filing Date: 25.10.2005
Chapter 2 Demand Filed: 29.03.2006

Int. Class.: C12N 15/00 (2006.01), C12M 1/34 (2006.01), C12Q 1/37 (2006.01), G01N 21/78 (2006.01)
Applicants: KYUSHU INSTITUTE OF TECHNOLOGY [JP/JP]; 1-1, Sensui-cho, Tobata-ku, Kitakyushu-shi Fukuoka, 8048550 (JP) (All Except US).
NISHINO, Norikazu [JP/JP]; 1-6-6, Hatakeda, Wakamatsu-ku, Kitakyushu-shi Fukuoka, 8080104 (JP) (US Only).
KATO, Tamaki [JP/JP]; 4-7-2-301, Sangamori, Yahatanishi-ku, Kitakyushu-shi Fukuoka, 8070843 (JP) (US Only).
Inventors: NISHINO, Norikazu [JP/JP]; 1-6-6, Hatakeda, Wakamatsu-ku, Kitakyushu-shi Fukuoka, 8080104 (JP).
KATO, Tamaki [JP/JP]; 4-7-2-301, Sangamori, Yahatanishi-ku, Kitakyushu-shi Fukuoka, 8070843 (JP).
Agent: ENOMOTO, Ichiro; Room 405, Kokurakosan-14-gokan,, 2-39, Asano 1-chome,, Kokurakita-ku, Kitakyushu-shi,, Fukuoka 802-0001 (JP).
Priority Data: 2005-006864 13.01.2005 JP
Abstract:
A particle for enzymatic activity detection that through measuring of the intensity of fluorescence wavelength of an analyte solution, realizes not only detection of the presence or absence of any enzyme but also quantitative analysis of any enzyme in the analyte solution with high accuracy, and that due to low susceptibility to adsorbed water and less occurrence of weighing error, excels in handleability and realizes enhanced quantitative determination with enhanced measuring accuracy, and that without the need to conduct complex steps, such as those of cleavage and purification of synthesized peptides and lyophilization thereof, in production, highly excels in productivity. There is provided particle for enzymatic activity detection (1) comprising particle (2) and first compound (3), the first compound (3) having its one end linked to the particle (2) and having its other end linked to first fluorescent group (4), the first compound (3) having a moiety at which cleavage by enzyme (5) contained in an analyte solution occurs.