Observation of salt-induced ?-lactoglobulin aggregation using sedimentation field-flow fractionation - Analytical Methods & Tools for Lyophilization

Observation of salt-induced ?-lactoglobulin aggregation using sedimentation field-flow fractionation
Engineering Village 2
2006 Elsevier Inc.
Accession number: 064710262263

Title: Observation of salt-induced ?-lactoglobulin aggregation using sedimentation field-flow fractionation

Authors: Saeseaw, Sudarat; Shiowatana, Juwadee; Siripinyanond, Atitaya

Author affiliation: Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Serial title: Analytical and Bioanalytical Chemistry

Abbreviated serial title: Anal. Bioanal. Chem.

Volume: v 386

Issue: n 6

Issue date: November 2006

Publication year: 2006

Pages: p 1681-1688

Language: English

ISSN: 1618-2642

CODEN: ABCNBP

Document type: Journal article (JA)

Publisher: Springer Verlag, Heidelberg, D-69121, Germany

Abstract: Sedimentation field-flow fractionation (SdFFF) was applied in order to characterize particle sizes of ?-lactoglobulin aggregates induced by Ca2+ or Zn2+. Aggregation induced by Zn2+ was faster than that induced by Ca2+. Effects of Zn2+ and ?-lactoglobulin concentrations, as well as contact time, on the aggregation of ?-lactoglobulin were examined. All factors exhibited a combined effect on the size of aggregates, whereby larger aggregates were obtained at increased concentrations of Zn2+ and ?-lactoglobulin. At fixed concentrations of 2% (w/v) ?-lactoglobulin and 10 mM Zn2+, the particle size of the aggregates increased from 0.19 ?m (at 15 min) to 0.38 ?m (at 2880 min). Further, a hyphenated technique of SdFFF and inductively coupled plasma-optical emission spectrometry (ICP-OES) was used to examine whether intermolecular ionic bridges take part in salt-induced ?-lactoglobulin aggregation. With SdFFF-ICP-OES, protein-cation-protein cross-linkages were observed for ?-lactoglobulin aggregation induced by Zn2+, but not for that induced by Ca2+. ? Springer-Verlag 2006.

Number of references: 31

Ei main heading: Proteins

Ei controlled terms: Agglomeration - Fractionation - Calcium - Zinc - Mass spectrometry - Concentration (process)

Uncontrolled terms: Lactoglobulin - Field-flow fractionation - Inductively coupled plasma-optical emission spectrometry - Calcium and zinc ions

Ei classification codes: 804.1 Organic Compounds - 802.3 Chemical Operations - 549.2 Alkaline Earth Metals - 546.3 Zinc & Alloys - 801 Chemistry

Treatment: Experimental (EXP)

DOI: 10.1007/s00216-006-0783-9

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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