Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation

Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation
Received for review March 2, 2004. Accepted April 15, 2004
Web Release Date: May 15, 2004
Dukjin Kang and Myeong Hee Moon*
Anal. Chem.
ACS Publications
Copyright ? 2004 American Chemical Society
Department of Chemistry, Yonsei University, Seoul, 120-749, Korea
A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 L/min, which reduces the injection amount of sample materials. The end effect contribution to plate height was evaluated by varying the inner diameter of the connection tubing between the injector and the channel inlet at various injection flow rates, and the results showed that the use of silica capillary tubing of the shortest possible distance is essential in reducing the initial band broadening prior to the sample injection to the microscale channel. The capability of the FI-AFlFFF system was demonstrated with the separation of protein standards, polystyrenesulfonates, and ssDNA strains and for the characterization of replication protein A-ssDNA binding complex regulated by redox status.
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