Method for the production of an N-terminally modified chemotactic factor - Patent Pending

United States Patent Application 20050148507
Kind Code A1
Wandl, Robert ; et al. July 7, 2005
Method for the production of an N-terminally modified chemotactic factor
Abstract
The invention relates to a process for preparing pyroGlu-MCP-1 from recombinantly produced Gln-MCP-1, wherein Gln-MCP-1 is incubated at a temperature in the range from 30.degree. C. and 80.degree. C. in a buffer solution with a salt concentration in the range from 10 mM to 160 mM and a pH in the range from 2 to 7.5, until at least 90% of the MCP-1 is present in the form of the pyroGlu-MCP-1.
Inventors: Wandl, Robert; (Vienna, AT) ; Necina, Roman; (Vienna, AT) ; Doods, Henri; (Warthausen, DE) ; Lenter, Martin; (Ulm, DE) ; Seidler, Randolph; (Sandy Hook, CT)
Correspondence Name and Address: MICHAEL P. MORRIS
BOEHRINGER INGELHEIM CORPORATION
900 RIDGEBURY ROAD
P. O. BOX 368
RIDGEFIELD
CT
06877-0368
US
Assignee Name and Adress: Boehringer Ingelheim International GmbH
Ingelheim
DE
Serial No.: 833656
Series Code: 10
Filed: April 28, 2004
U.S. Current Class: 514/12; 530/350
U.S. Class at Publication: 514/012; 530/350
Intern'l Class: A61K 038/17; C07K 014/475