Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation

Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation
Engineering Village 2
2006 Elsevier Inc.
Accession number: 9078693

Title: Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation

Authors: Brown, P.H.1 ; Schuck, P.1

Author affiliation: 1 Div. of Bioeng. & Phys. Sci., Nat. Inst. of Health, Bethesda, MD, USA

Serial title: Biophysical Journal

Abbreviated serial title: Biophys. J. (USA)

Volume: 90

Issue: 12

Publication date: 15 June 2006

Pages: 4651-61

Language: English

ISSN: 0006-3495


Document type: Journal article (JA)

Publisher: Biophys. Soc

Country of publication: USA

Material Identity Number: B154-2006-012

Abstract: Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particles, determined from the experimental data, which scales the diffusion coefficient to the sedimentation coefficient consistent with the traditional s ~ M2/3 power law. It provides a high hydrodynamic resolution, where diffusional broadening of the sedimentation boundaries is deconvoluted from the sedimentation coefficient distribution. The approximation of a single weight-average frictional ratio is favored by several experimental factors, and usually gives good results for chemically not too dissimilar macromolecules, such as mixtures of folded proteins. In this communication, we examine an extension to a two-dimensional distribution of sedimentation coefficient and frictional ratio, c(s,fr), which is representative of a more general set of size-and-shape distributions, including mass-Stokes radius distributions, c(M,RS), and sedimentation coefficient-molar mass distributions c(s,M). We show that this can be used to determine average molar masses of macromolecules and characterize macromolecular distributions, without the approximation of any scaling relationship between hydrodynamic and thermodynamic parameters

Number of references: 42

Inspec controlled terms: biological techniques - biomechanics - friction - macromolecules - molecular biophysics - proteins - sedimentation

Uncontrolled terms: macromolecular size distribution - macromolecular shape distribution - sedimentation velocity analytical ultracentrifugation - sedimentation coefficient distribution - Lamm equation solutions - single weight-average frictional coefficient - diffusion coefficient - diffusional broadening - sedimentation boundaries - single weight-average frictional ratio - folded proteins - mass-Stokes radius distributions - sedimentation coefficient-molar mass distributions - macromolecular distributions

Inspec classification codes: A8780 Biophysical instrumentation and techniques - A8715 Molecular biophysics - A8745 Biomechanics, biorheology, biological fluid dynamics - A8270 Disperse systems

Treatment: Practical (PRA); Experimental (EXP)

Discipline: Physics (A)

DOI: 10.1529/biophysj.106.081372

Database: Inspec

Copyright 2006, The Institution of Engineering and Technology
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