METHOD FOR PRODUCING RIBONUCLEOTIDES - European Patents

Patent number: UA66706
Publication date: 2004-05-17
Inventor: TEMIROV YURII PAVLOVYCH (UA); SHVETS VITALII IVANOVYCH (RU); KRASNOPOLSKYI YURII MYKHAILOVY (UA)
Applicant: JOINT STOCK COMPANY KHARKIV EN (UA)
Classification:
- international: A61K37/00; A61K31/785; C12P19/34
- european:
Application number: UA20030109081 20031007
Priority number(s): UA20030109081 20031007
Abstract of UA66706
The method for producing ribonucleotides provides for hydrolysis of yeast ribonucleic acid in solution by pancreatic ribonuclease, precipitation by ethanol, ultrafiltration, treatment with 8-10-fold volume of ethanol, drying of the precipitate, its dissolution in sodium chloride solution, the sterilizing filtration, filling of the ampoules, and the sterilization. The hydrolysis is performed for 8-10 hours. The precipitation by ethanol is performed at the temperature of 8-12 DEGREE c. The precipitate is freeze-dried. The sterilization is carried out at a temperature of 110-112 DEGREE c for 8-10 minutes.
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