METHOD FOR IMPROVING STABILITY AND SHELF-LIFE OF LIPOSOME COMPLEXES

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METHOD FOR IMPROVING STABILITY AND SHELF-LIFE OF LIPOSOME COMPLEXES
Publication Number: WO/2005/000271 International Application No.: PCT/US2004/017695
Publication Date: 06.01.2005 International Filing Date: 04.06.2004

Int. Class.: A61K 47/48 (2006.01), A61K 48/00 (2006.01), A61K 49/00 (2006.01), C12N 15/88 (2006.01)
Applicants: GEORGETOWN UNIVERSITY [US/US]; 37th & O Streets, N.W., Washington, DC 20057 (US) (All Except US).
CHANG, Esther, H. [US/US]; 10244 Democracy Blvd., Potomac, MA 20854 (US) (US Only).
PIROLLO, Kathleen F. [US/US]; 547 Anderson Avenue, Rockville, MA 20850 (US) (US Only).
Inventors: CHANG, Esther, H. [US/US]; 10244 Democracy Blvd., Potomac, MA 20854 (US).
PIROLLO, Kathleen F. [US/US]; 547 Anderson Avenue, Rockville, MA 20850 (US).
Agent: ERNST, Barbara, G.; 1425 K Street, N.W., Suite 800, Washington, DC 20005 (US).
Priority Data: 60/475,500 04.06.2003 US

Abstract: A method for preparing a stable cell-targeting complex comprising a ligand and a cationic liposome encapsulating a therapeutic or diagnostic agent comprises (a) combining the complex with a solution comprising a stabilizing amount of sucrose and (b) lyophilizing the resultant solution to obtain a lyophilized preparation; wherein, upon reconstitution, the preparation retains at least about 80% of its pre-lyophilization activity.