Lyophilizable and enhanced compacted nucleic acids

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United States Patent Application 20020042388
Kind Code A1
Cooper, Mark J. ; et al. April 11, 2002
Abstract
Counterions of polycations used to compact nucleic acids profoundly affect shape and stability of particles formed. Shape is associated with differential serum nuclease resistance and colloidal stability. A surrogate for determining such properties that is easy to measure is the turbidity parameter. Shape also affects the suitability and efficacy of compacted nucleic acid complexes for transfecting cells by various routes into a mammalian body. Moreover, counterions such as acetate can protect compacted nucleic acid complexes from adverse effects of lyophilization
Inventors: Cooper, Mark J.; (Moreland Hills, OH) ; Pasumarthy, Murali K.; (Twinsburg, OH) ; Kowalczyk, Tomasz H.; (University Heights, OH) ; Costello, Maureen; (Margate City, NJ)
Correspondence Name and Address: BANNER & WITCOFF
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Serial No.: 867693
Series Code: 09
Filed: May 31, 2001
U.S. Current Class: 514/44; 435/6
U.S. Class at Publication: 514/44; 435/6
Intern'l Class: A61K 048/00; C12Q 001/68