LIPOSOMES CONTAINING PARTICULATE MATERIALS - Patent Pending

12) Patent Application: (11) CA 2173601
ABSTRACT:
A method is provided for the formation of liposomes of 0.1 ?m to 50 ?m in diameter having unilamella or multilamella structure and containing water insoluble or undissolved particulate materials comprising
(a) forming liposomes and removing substantially all of any organic solvent used in their preparation, (b) freeze drying the liposomes so formed and then (c) rehydrating them in intimate admixture with the particulate material. Preferred encapsulated materials are particulate materials, most preferably microorganisms, plant or animal cells or water insoluble structures having organic solvent labile biochemical or immunological activity, but any water insoluble particulate may be encapsulated using the method. For example catalysts or drugs that are sparingly soluble may also be so incorporated such that slow release into the patient's body may be provided while release of detergents included in the many liposome preparation protocols may be avoided.
(72) Inventors (Country): GREGORIADIS, GREGORY (U.K.)
ANTIMISIARIS, SOPHIA GEORGE (Greece)
GURSEL, IHSAN (Turkey)
(73) Owners (Country): THE SECRETARY OF STATE FOR DEFENCE IN HER BRITANNIC MAJESTY'S GOVERNMENT OF THE UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND (U.K.)
(71) Applicants (Country):
(74) Agent: FETHERSTONHAUGH & CO.
(45) Issued:
(86) PCT Filing Date: Oct. 7 , 1994
(87) PCT Publication Date: Apr. 13, 1995
Examination requested: Aug. 3 , 2001
(51) International Class (IPC): B01J 13/02
C12N 11/04
A61K 9/127
Patent Cooperation Treaty (PCT): Yes (85) National Entry: Apr. 4 , 1996
(86) PCT Filing number: PCT/GB1994/002191
(87) International publication number: WO1995/009610
(30) Application priority data: Application No. Country Date
9320668.8 U.K. Oct. 7 , 1993
Availability of licence: N/A
Language of filing: English