Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Gu?rin

Ion-exchange chromatography method for the purification of genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Gu?rin
25 November 2004
Received 13 October 2003; accepted 15 June 2004. Available online 1 October 2004.
Journal of Chromatography B
Volume 811, Issue 2
ScienceDirect
aDepartment of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China
bDepartment of Genetics, The Second Military Medical University, Shanghai 200433, China
Abstract
The goal of this study was to provide practical strategies for purifying genomic DNA fraction from Mycobacterium bovis Bacillus Calmette-Gu?rin (BCG-DNA) by ion-exchange chromatography. A multistep process was developed to purify BCG-DNA. The process consisted of sonication, heating, trypsin digestion, ion-exchange chromatography, gel-filter chromatography, and lyophilization. After ion-exchange chromatography, BCG-DNA was highly purified and possessed potent biological effects. The methods described were efficient and had good reproducibility. Further, this was the first reported chromatography method to purify BCG-DNA.
Keywords: Purification; DNA

Corresponding author. Tel.: +86 21 25070331.
This is a subscription site. You will need to register and pay to view the Full Text Article.