In vitro determination of normal and neoplastic human brain tissue optical properties using inverse adding-doubling

In vitro determination of normal and neoplastic human brain tissue optical properties using inverse adding-doubling
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 8975972

Title: In vitro determination of normal and neoplastic human brain tissue optical properties using inverse adding-doubling

Authors: Gebhart, S.C.1 ; Lin, W.C.; Mahadevan-Jansen, A.

Author affiliation: 1 Dept. of Biomed. Eng., Vanderbilt Univ., Nashville, TN, USA

Serial title: Physics in Medicine and Biology

Abbreviated serial title: Phys. Med. Biol. (UK)

Volume: 51

Issue: 8

Publication date: 21 April 2006

Pages: 2011-27

Language: English

ISSN: 0031-9155


Document type: Journal article (JA)

Publisher: IOP Publishing

Country of publication: UK

Material Identity Number: P117-2006-012

Abstract: To complement a project towards the development of real-time optical biopsy for brain tissue discrimination and surgical resection guidance, the optical properties of various brain tissues were measured in vitro and correlated to features within clinical diffuse reflectance tissue spectra measured in vivo. Reflectance and transmission spectra of in vitro brain tissue samples were measured with a single-integrating-sphere spectrometer for wavelengths 400-1300 nm and converted to absorption and reduced scattering spectra using an inverse adding-doubling technique. Optical property spectra were classified as deriving from white matter, grey matter or glioma tissue according to histopathologic diagnosis, and mean absorption and reduced scattering spectra were calculated for the three tissue categories. Absolute reduced scattering and absorption values and their relative differences between histopathological groups agreed with previously reported results with the exception that absorption coefficients were often overestimated, most likely due to biologic variability or unaccounted light loss during reflectance/transmission measurement. Absorption spectra for the three tissue classes were dominated by haemoglobin absorption below 600 nm and water absorption above 900 nm and generally determined the shape of corresponding clinical diffuse reflectance spectra. Reduced scattering spectral shapes followed the power curve predicted by the Rayleigh limit of Mie scattering theory. While tissue absorption governed the shape of clinical diffuse reflectance spectra, reduced scattering determined their relative emission intensities between the three tissue categories

Number of references: 34

Inspec controlled terms: bio-optics - biological tissues - biomedical measurement - brain - light transmission - Mie scattering - molecular biophysics - patient diagnosis - proteins - Rayleigh scattering - reflectivity

Uncontrolled terms: normal human brain tissue - neoplastic human brain tissue - optical properties - inverse adding-doubling technique - real-time optical biopsy - brain tissue discrimination - surgical resection guidance - clinical diffuse reflectance tissue spectra - transmission spectra - single-integrating-sphere spectrometer - absorption spectra - reduced scattering spectra - white matter - gray matter - glioma tissue - histopathologic diagnosis - hemoglobin absorption - water absorption - Rayleigh limit - Mie scattering theory - tissue absorption

Inspec classification codes: A8760F Optical and laser radiation (medical uses) - A8750B Interactions of biosystems with radiations - A8770E Patient diagnostic methods and instrumentation - A8715M Interactions with radiations at the biomolecular level - B7510J Optical and laser radiation (biomedical imaging/measurement)

Treatment: Practical (PRA); Experimental (EXP)

Discipline: Physics (A); Electrical/Electronic engineering (B)

DOI: 10.1088/0031-9155/51/8/004

Database: Inspec

Copyright 2006, The Institution of Engineering and Technology
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