In vacuo glycation of proteins - Patent Pending

In vacuo glycation of proteins
Engineering Village
Publication number: US20060122369

Application number: 10524547

Unstandardized application number: 524547

Patent authority: US

Kind: A1 - Utility Patent Application

Title: In vacuo glycation of proteins

Inventors: Kaplan, Harvey (CA) ; King, Mary Catherine (CA) ; Stewart, Nicolas Andre Stirling (CA)

Publication date: 06/08/2006

Publication year: 2006

Filing date: 08/15/2003

Abstract: It has been discovered that facile glycation of proteins can be achieved by colyophilization of a protein with a reducing sugar, subjecting the lyophilized mixture to a vacuum (10 to 50 millitor) and incubating at an elevated temperature (50 to 100? C.) for 1 to 24 h. A stable ketoamine derivative is formed with amino groups in the protein and no advanced glycation end products (browning reaction) are observed, as is the case with aqueous glycation procedures. Another novel feature is that the in vacuo glycation reaction takes place with the protonated amine and not the deprotonated amine as is believed to be the case for aqueous glycation reactions. Advantage can be taken of the in vacuo glycation reaction to achieve facile covalent cross-linking of proteins by lyophilizing protein or proteins with compounds containing two or more reducing sugars separated by a linker.

Document type: US Application

Priority information: CA 2398213 08/15/2002

IPC-8 Code: C07K 9/00 SECTION C CHEMISTRY; METALLURGY
ORGANIC CHEMISTRY such compounds as the oxides, sulfides, or oxysulfides of carbon, cyanogen, phosgene, hydrocyanic acid or salts thereof ; products obtained from layered base-exchange silicates by ion-exchange with organic compounds such as ammonium, phosphonium or sulfonium compounds or by intercalation of organic compounds ; macromolecular compounds ; dyes ; fermentation products ; fermentation or enzyme-using processes to synthesise a desired chemical compound or composition or to separate optical isomers from a racemic mixture ; production of organic compounds by electrolysis or electrophoresis , 2
PEPTIDES peptides in foodstuffs , e.g. obtaining protein compositions for foodstuffs ; preparations for medicinal purposes ; peptides containing -lactam rings ; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, ; ergot alkaloids of the cyclic peptide type ; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific, but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids ; macromolecular products derived from proteins ; preparation of glue or gelatine ; single cell proteins, enzymes ; genetic engineering processes for obtaining peptides ; compositions for measuring or testing processes involving enzymes ; investigation or analysis of biological material 4
Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof 4,6

US Classification: 530/395 CHEMISTRY: NATURAL RESINS OR DERIVATIVES; PEPTIDES OR PROTEINS; LIGNINS OR REACTION PRODUCTS THEREOF
PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES
Glycoprotein, e.g., mucins proteoglycans, etc.

530/322 CHEMISTRY: NATURAL RESINS OR DERIVATIVES; PEPTIDES OR PROTEINS; LIGNINS OR REACTION PRODUCTS THEREOF
PEPTIDES OF 3 TO 100 AMINO ACID RESIDUES
Peptides containing saccharide radicals, e.g., bleomycins, etc.

536/55.3 ORGANIC COMPOUNDS -- PART OF THE CLASS 532-570 SERIES
ORGANIC COMPOUNDS (CLASS 532, SUBCLASS 1)
Carbohydrates or derivatives
Nitrogen containing
Processes

Database: US Patents

Compilation and indexing terms, ? 2006 LexisNexis Univentio B.V.

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