HUMAN G-PROTEIN CHEMOKINE RECEPTOR HDGNR10 - European Patents

HUMAN G-PROTEIN CHEMOKINE RECEPTOR HDGNR10
Publication number: CA2562211
Publication date: 1996-12-12
Inventor: RUBEN STEVEN M (US); LI YI (US)
Applicant: HUMAN GENOME SCIENCES INC (US)
Classification:
- international: C12N15/19; A61K38/19; C07H21/00; C07K14/715; C07K16/28; C12N1/21; C12N5/10; C12N15/63; C12P21/02; C12Q1/68; G01N33/566; C12N15/19; A61K38/19; C07H21/00; C07K14/435; C07K16/18; C12N1/21; C12N5/10; C12N15/63; C12P21/02; C12Q1/68; G01N33/566;
- European:
Application number: CA19952562211 19950606
Priority number(s): CA19952216912 19950606

Abstract of CA2562211

Human G-protein chemokine receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein chemokine receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein chemokine receptor nucleic acid sequences and detecting a level of the soluble form of the receptors in a sample derived from a host. <SDOCL LA=EN> THE EMBODIMENTS OF THE INVENTION FOR WHICH AN EXCLUSIVE PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS: 1. A plasmid encoding HDGNR10 as contained in ATCC Deposit No. 97183. 2. An isolated polynucleotide comprising a nucleic acid encoding a protein which is at least 90% identical to the human G-protein chemokine receptor polypeptide encoded by the clone in ATCC Deposit No. 97183. 3. The polynucleotide of claim 2, wherein said protein is at least 95% identical to the human G-protein chemokine receptor polypeptide encoded by the clone in ATCC Deposit No. 97183. 4. The polynucleotide of claim 2, wherein said protein is identical to the human G- protein chemokine receptor polypeptide encoded by the clone in ATCC Deposit No. 97183. 5. An isolated polynucleotide comprising a nucleic acid encoding a protein which is at least 90% identical to a mature form of the human G-protein chemokine receptor polypeptide encoded by the clone in ATCC Deposit No. 97183. 6. The polynucleotide of claim 5, wherein said protein is at least 95% identical to a mature form of the human G-protein chemokine receptor polypeptide encoded by the clone in ATCC Deposit No. 97183. 7. The polynucleotide of claim 5, wherein said protein is identical to a mature form of the human G-protein chemokine receptor polypeptide encoded by the clone i n ATCC Deposit No. 97183. 8. An isolated polynucleotide comprising a nucleic acid encoding an amino ac id sequence that is a fragment of the human G-protein chemokine receptor polypeptide encoded by the clone in ATCC Deposit No. 97183. 51 9. An isolated polynucleotide which is the complement of the isolated polynucleotide of any one of claims 1 to 8. 10. A vector comprising the polynucleotide of any one of claims 1 to 9. 11. A host cell comprising the vector of claim 10. 12. A human G-protein chemokine receptor polypeptide or fragment thereof encoded by the polynucleotide of any one of claims 1 to 8. 13. A pharmaceutical composition comprising the human G-protein chemokine receptor polypeptide or fragment thereof of claim 12. 14. An isolated polynucleotide comprising a nucleic acid encoding a protein which is at least 90% identical to the human G-protein chemokine receptor polypeptide consisting of the amino acid sequence as set forth in SEQ ID NO:2. 15. An isolated polynucleotide comprising a nucleic acid encoding a protein which is at least 90% identical to a mature form of the human G-protein chemokine receptor polypeptide consisting of the amino acid sequence as set forth in SEQ ID NO: 2. 16. An isolated polynucleotide which is the complement of the isolated polynucleotide of claim 14 or 15 or a fragment thereof. 17. A vector comprising the polynucleotide of claim 14 or 15. 18. A host cell transformed or transfected with the vector of claim 17. 19. A process for producing a polypeptide comprising: expressing from the ho st cell of claim 18 the polypeptide encoded by said polynucleotide. 20. A process for producing cells capable of expressing a polypeptide comprising transforming or transfecting the cells with the vector of claim 17. 52 21. A human G-protein chemokine receptor polypeptide or fragment thereof encoded by the polynucleotide of claim 14 or 15. 22. The human G-protein chemokine receptor polypeptide or fragment thereof o f claim 2I, wherein the polypeptide or fragment thereof has the deduced amino acid sequence of SEQ ID NO:2, or a fragment thereof. 23. A pharmaceutical composition comprising the human G-protein chemokine receptor polypeptide or fragment thereof of claim 21 or 22. 53</SDOCL>