Glycoforms of ?-Lactoglobulin with Improved Thermostability and Preserved Structural Packing

Glycoforms of ?-Lactoglobulin with Improved Thermostability and Preserved Structural Packing
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 04138084112

Title: Glycoforms of ?-Lactoglobulin with Improved Thermostability and Preserved Structural Packing

Authors: Broersen, Kerensa; Voragen, Alphons G. J.; Hamer, Rob J.; De Jongh, Harmen H. J.

Author affiliation: Wageningen Centre for Food Sciences, 6700 AN Wageningen, Netherlands

Serial title: Biotechnology and Bioengineering

Abbreviated serial title: Biotechnol. Bioeng.

Volume: v 86

Issue: n 1

Issue date: Apr 5 2004

Publication year: 2004

Pages: p 78-87

Language: English

ISSN: 0006-3592


Document type: Journal article (JA)

Publisher: John Wiley and Sons Inc.

Abstract: In this article we show how various degrees of glycosylation can be used to control the thermal stability of proteins. The primary amines of ?-lactoglobulin were glycosylated with glucose or fructose within a range of non-denaturing reaction parameters. The modified fractions were characterized and analyzed for structural stability and hydrophobic exposure. The modification procedure gave rise to the production of glycoproteins with a well-defined Gaussian distribution, where glucose appeared more reactive than fructose. The integrity of the secondary, tertiary, and quaternary structures remained unaffected by the modification procedure. However, upon heating the stability of the modified fractions increased up to 6 K. Here we demonstrate the effects on the thermodynamic properties of proteins by glycosylation; this work serves as a first step in understanding and controlling the process underlying aggregation of glycosylated proteins. ? 2004 Wiley Periodicals, Inc.

Number of references: 45

Ei main heading: Biotechnology

Ei controlled terms: Proteins - Amines - Glucose - Hydrophobicity - Thermodynamics

Uncontrolled terms: Thermostability - Structural stability

Ei classification codes: 461.8 Biotechnology - 804.1 Organic Compounds - 931.2 Physical Properties of Gases, Liquids & Solids - 641.1 Thermodynamics

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1002/bit.20030

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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