Glutaraldehyde-treated Bovine Pericardium: Effects of Lyophilization on Cytotoxicity and Residual Aldehydes

Glutaraldehyde-treated Bovine Pericardium: Effects of Lyophilization on Cytotoxicity and Residual Aldehydes
August 2003
*Marina J.S. Maizato, Olga Z. Higa, M?nica B. Mathor, Maria A.P. Camillo, Patric J. Spencer, Ronaldo N. Pitombo, ?Cec?lia A.C. Zavaglia, and *Adolfo A. Leirner
Artificial Organs
Volume 27 Issue 8 Page 692
Blackwell Synergy
*Heart Institute (InCor), University of S?o Paulo Medical School; Instituto de Pesquisas Energ?ticas e Nucleares; Departamento de Tecnologia Bioqu?mico-Farmac?utica da Faculdade de Farm?cia da Universidade de S?o Paulo; and ?Departamento de Materiais da Faculdade de Engenharia Mec?nica da Universidade Estadual de Campinas (DEMA/FEM/UNICAMP), S?o Paulo, Brazil
Abstract:
This work assesses the effect of lyophilization on the cytotoxicity and residual aldehyde concentra- tion of glutaraldehyde-treated and lyophilized bovine pericardium (group A), comparing it to conventional glutaraldehyde-treated bovine pericardium (group B). Cytotoxicity was measured by incubating a pericardium sample from each group in saline and assessing the eluant's influence on cellular growth. Residual aldehydes were measured by HPLC. Although both groups' eluants exhibited some cytotoxicity, the eluant from group A was less cytotoxic, with a cytotoxicity index (IC50(%)) of 41%. Group B eluants all had marked cytotoxic effects; cell growth was 24.15% of the negative control at the most dilute eluant concentration (6.25%). The mean residual glutaraldehyde level was less in group A than in group B (2.36 ? 0.11 and 9.90 ? 3.70 g/l, respectively; n=3, P< 0.05), but residual formaldehyde levels did not differ. These results demonstrate that compared with conventional glutaraldehyde-treated bovine pericardium, lyophilized pericardium is less cytotoxic, with fewer glutaraldehyde residues.
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