Field-flow fractionation of proteins polysaccharides, synthetic polymers, and supramolecular assemblies - Analytical Methods & Tools for Lyophilization

Field-flow fractionation of proteins polysaccharides, synthetic polymers, and supramolecular assemblies
Engineering Village 2
2006 Elsevier Inc.
Accession number: 063710109359

Title: Field-flow fractionation of proteins polysaccharides, synthetic polymers, and supramolecular assemblies

Authors: Ratanathanawongs Williams, S. Kim; Lee, Dean

Author affiliation: Department of Chemistry and Geochemistry, Colorado School of Mines, Golden, CO 80401, United States

Serial title: Journal of Separation Science

Abbreviated serial title: J. Sep. Sci.

Volume: v 29

Issue: n 12

Issue date: August 2006

Publication year: 2006

Pages: p 1720-1732

Language: English

ISSN: 1615-9306

CODEN: JSSCCJ

Document type: Journal article (JA)

Publisher: Wiley-VCH Verlag, Weinheim, D-69451, Germany

Abstract: This review summarizes developments and applications of flow and thermal field-flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particles, and block copolymers have been characterized and processes such as aggregation, stability, and infectivity have been monitored. The open channel design used in FFF makes it a gentle separation technique for high- and ultrahigh-molecular weight macromolecules, aggregates, and self-assembled complexes. Coupling FFF with other techniques such as multiangle light scattering and MS provides additional invaluable information about conformation, branching, and identity. ? 2006 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.

Number of references: 126

Ei main heading: Fractionation

Ei controlled terms: Proteins - Polysaccharides - Supramolecular chemistry - Macromolecules - Self assembly - Nanostructured materials - Agglomeration - Conformations

Uncontrolled terms: Field-flow fractionation - Protein aggregates - Supramolecular assemblies

Ei classification codes: 802.3 Chemical Operations - 804.1 Organic Compounds - 801.4 Physical Chemistry - 931.3 Atomic & Molecular Physics - 933.1 Crystalline Solids

Treatment: Experimental (EXP)

DOI: 10.1002/jssc.200600151

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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