Field-flow fractionation and biotechnology

Field-flow fractionation and biotechnology
Available online 2 August 2005
Pierluigi Reschigliana, Andrea Zattonia, Barbara Rodaa, Elisa Michelinib and Aldo Rodab
Trends in Biotechnology
Volume 23, Issue 9 , September 2005
Copyright ? 2005 Elsevier Ltd All rights reserved.
aDepartment of Chemistry ?G. Ciamician?, University of Bologna, Via Selmi 2, I-40126 Bologna, Italy
bDepartment of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, I-40126 Bologna, Italy
The gentle separation mechanism has made field-flow fractionation particularly suited to samples of biotechnological interest, from proteins and nucleic acids to viruses, subcellular units and whole cells. Recent progress in field-flow fractionation technology, as well as the development of coupled techniques combining field-flow fractionation capabilities with the specificity and sensitivity of well-established analytical methods, opens up new biotechnological applications for field-flow fractionation. The most recent appealing applications include: sorting and fingerprinting of bacteria for whole-cell vaccine production; noninvasive and tagless sorting of immature and stem cells; separation of intact proteins and enzymes in top-down proteomics; and the development of flow-assisted, multianalyte immunoassays using nano- and micron-sized particles with immobilized biomolecules.
You can view the abstract online. A subscription is required to view the full text or it can be purchased online.
Comments: 0