Evaluation of thermodynamic properties of irreversible protein thermal unfolding measured by DSC

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Evaluation of thermodynamic properties of irreversible protein thermal unfolding measured by DSC
Engineering Village 2
? 2006 Elsevier Inc
Accession number: 05209106558

Title: Evaluation of thermodynamic properties of irreversible protein thermal unfolding measured by DSC

Authors: Manetto, G.D.; La Rosa, C.; Grasso, D.M.; Milardi, D.

Author affiliation: CNR, Ist. Di Biostrutture E B., 95125 Catania, Italy

Serial title: Journal of Thermal Analysis and Calorimetry

Abbreviated serial title: J Therm Anal Calor

Volume: v 80

Issue: n 2

Issue date: May 2005

Publication year: 2005

Pages: p 263-270

Language: English

ISSN: 1388-6150

CODEN: JTACF7

Document type: Journal article (JA)

Publisher: Kluwer Academic Publishers, Dordrecht, 3311 GZ, Netherlands

Abstract: We assessed the applicability of the extrapolation procedure at infinite scanning rate to differential scanning calorimetry (DSC) data related to irreversible protein unfolding. To this aim, an array of DSC curves have been simulated on the basis of the Lumry-Eyring model N and harr;U and rarr;F. The results obtained confirmed that when the apparent equilibrium constant K app (T=T1/2) is lower than 3, the application of the extrapolation procedure provides accurate thermodynamic parameters. Although this procedure applies only to monomeric proteins for which the Lumry-Eyring model is a reasonable approximation, it will hopefully contribute to increase the potential of DSC in obtaining reliable thermodynamic information regarding the folding/unfolding equilibrium. ? 2005 Akademiai Kiado, Budapest.

Number of references: 32

Ei main heading: Proteins

Ei controlled terms: Thermodynamic properties - Differential scanning calorimetry - Monomers - Specific heat - Reliability - Kinetic energy - Enthalpy - Equations of motion - Extrapolation - Mathematical models

Uncontrolled terms: Extrapolation to infinite scan rate - Thermal unfolding - Time-dependent factors - Protein folding-unfolding transitions

Ei classification codes: 804.1 Organic Compounds - 641.1 Thermodynamics - 944.6 Temperature Measurements - 931.1 Mechanics - 921.2 Calculus - 921.6 Numerical Methods

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1007/s10973-005-0646-1

Database: Compendex

Compilation and indexing terms, ? 2006Elsevier Inc. All rights reserved
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