Elution behavior of protein and pullulan in asymmetrical flow field-flow fractionation (AsFlFFF)

Elution behavior of protein and pullulan in asymmetrical flow field-flow fractionation (AsFlFFF)
Engineering Village 2
2006 Elsevier Inc.
Accession number: 064010143409

Title: Elution behavior of protein and pullulan in asymmetrical flow field-flow fractionation (AsFlFFF)

Authors: Ji, Eunsun; Choi, Seong-Ho; Kuk, Ro Yoon; Chun, Jong-Han; Lee, Seungho

Author affiliation: Department of Chemistry, Hannam University, Daejeon 306-791, South Korea

Serial title: Bulletin of the Korean Chemical Society

Abbreviated serial title: Bull. Korean Chem. Soc.

Volume: v 27

Issue: n 9

Issue date: Sep 20 2006

Publication year: 2006

Pages: p 1433-1438

Language: English

ISSN: 0253-2964


Document type: Journal article (JA)

Publisher: Korean Chemical Society, Seoul, 135-703, South Korea

Abstract: An AsFlFFF channel was designed and built, and then tested for analysis of pullulans and proteins. Pullulans and proteins having various nominal molecular weights were injected at various conditions of the cross-flow rate (F c) and the channel-out flow rate (Fout). The retention (measured by the retention ratio R) and the zone broadening (measured by the plate height H) were measured, and then compared with theory. When the incoming flow rate, Fin (and thus Fout) was varied with F c fixed at 2.5 mL/min, the plate height measured for the pullulan with nominal molecular weight (M) of about 100,000 showed the trend expected by the "longitudinal diffusion" theory (H decreases with increasing flow rate). In contrast, when Fout was varied with the flow rate ratio, Fc/Fout, fixed constant at 5, the plate height measured for the same sample showed the trend expected from the "non- equilibrium" theory (H increases with increasing flow rate). Calibration plots (log D vs. log M) obtained with pullulans and proteins were not coincide, probably due to the difference in molecular conformation, suggesting the analysis of pullulans and proteins using AsFlFFF requires independent calibration. It was found that the linearity of the protein-calibration plot was improved by using a buffer solution as the carrier.

Number of references: 23

Ei main heading: Proteins

Ei controlled terms: Polysaccharides - Molecular weight - Fluid dynamics - Conformations - Molecular dynamics - Calibration

Uncontrolled terms: Asymmetrical flow-field flow fractionation (AsFlFFF) - Pullulan - Plate height - Buffer solution

Ei classification codes: 804.1 Organic Compounds - 815.1.1 Organic Polymers - 931.3 Atomic & Molecular Physics - 931.1 Mechanics - 801.4 Physical Chemistry - 902.2 Codes & Standards

Treatment: Theoretical (THR); Experimental (EXP)

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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