Diffusion NMR as a new method for the determination of the gel point of gelatin - Analytical Methods & Tools for Lyophilization

Diffusion NMR as a new method for the determination of the gel point of gelatin
Engineering Village 2
2006 Elsevier Inc.
Accession number: 063810117317

Title: Diffusion NMR as a new method for the determination of the gel point of gelatin

Authors: Brand, Torsten; Richter, Sven; Berger, Stefan

Author affiliation: Institute of Analytical Chemistry, University of Leipzig, D-04103 Leipzig, Germany

Serial title: Journal of Physical Chemistry B

Abbreviated serial title: J Phys Chem B

Volume: v 110

Issue: n 32

Issue date: Aug 17 2006

Publication year: 2006

Pages: p 15853-15857

Language: English

ISSN: 1520-6106

CODEN: JPCBFK

Document type: Journal article (JA)

Publisher: American Chemical Society, Columbus, OH 43210-3337, United States

Abstract: The gelation of gelatin has been investigated using pulsed field gradient (PFG) NMR. For the first time, diffusion results have been used to determine the gelation point, which is indicated by a minimum in the self-diffusion coefficient of the free polymer fraction vs temperature. Biexponential analysis of the diffusion decay data allowing the diffusion of free and network-bound gelatin to be determined separately has been applied to provide an extended insight into the gelation process. Low-amplitude oscillatory shear rheology and time-resolved dynamic light scattering (DLS) as classical polymer characterization methods were applied as control experiments. All three methods yielded a gelation temperature of 24-25 ?C for the cooling regime. Hysteresis effects could also be observed. ? 2006 American Chemical Society.

Number of references: 35

Ei main heading: Proteins

Ei controlled terms: Diffusion - Nuclear magnetic resonance - Gels - Light scattering - Rheology

Uncontrolled terms: Biexponential analysis - Diffusion decay data - Self-diffusion coefficients - Dynamic light scattering (DLS)

Ei classification codes: 804.1 Organic Compounds - 931.1 Mechanics - 931.2 Physical Properties of Gases, Liquids & Solids - 801.3 Colloid Chemistry - 741.1 Light/Optics

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1021/jp062960z

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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