Detection of proteins by on-column, non-covalent labeling with NanoOrange during capillary zone electrophoresis
Detection of proteins by on-column, non-covalent labeling with NanoOrange during capillary zone electrophoresis
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06209879926
Title: Detection of proteins by on-column, non-covalent labeling with NanoOrange during capillary zone electrophoresis
Authors: Feng, Hua-Tao; Li, Sam Fong-Yau; Chia, Pei-Shan; Loke, Weng-Keong
Author affiliation: Department of Chemistry, National University of Singapore, Singapore, 117543, Singapore
Serial title: Journal of Liquid Chromatography and Related Technologies
Abbreviated serial title: J. Liq. Chromatogr. Relat. Technol.
Volume: v 29
Issue: n 10
Issue date: Jul 1 2006
Publication year: 2006
Pages: p 1447-1455
Language: English
ISSN: 1082-6076
CODEN: JLCTFC
Document type: Journal article (JA)
Publisher: Taylor and Francis Inc., Philadelphia PA, PA 19106, United States
Abstract: To avoid prior derivatization of proteins with fluorescent reagents, oncolumn labeling of non-covalent dye NanoOrange on proteins was evaluated with sodium dodecyl sulfate (SDS) as an additive using laser induced fluorescence (LIF) monitoring in capillary electrophoresis (CE). Performance of NanoOrange in buffer solutions of various pH values was tested. Acidic buffer solution was found to be unsuitable for on-column NanoOrange labeling. SDS enhanced the fluorescence intensities of basic proteins with high pI values. The fluorescence intensities of protein solutions with or without SDS additives were compared using a fluorescence spectrophotometer. The applicability of on-column labeling and detection of staphylococcal enterotoxin B was also demonstrated. This on-column labeling method provides a rapid, direct, widely applicable technique for protein studies. Copyright ? Taylor and Francis Group, LLC.
Number of references: 23
Ei main heading: Proteins
Ei controlled terms: Electrophoresis - Capillarity - Capillary flow - pH effects - Fluorescence - Spectrophotometers
Uncontrolled terms: Capillary electrophoresis - Laser induced fluorescence - NanoOrange - Noncovalent labeling
Ei classification codes: 804.1 Organic Compounds - 701.1 Electricity: Basic Concepts & Phenomena - 631.1 Fluid Flow, General - 801.1 Chemistry, General - 741.1 Light/Optics - 741.3 Optical Devices & Systems
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1080/10826070600674844
Database: Compendex
Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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