Continuous-flow pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential
Continuous-flow pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential
Engineering Village 2
2006 Elsevier Inc.
Accession number: 06249937999
Title: Continuous-flow pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential
Authors: Song, Yong-Ak; Hsu, Stephanie; Stevens, Anna L.; Han, Jongyoon
Author affiliation: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Serial title: Analytical Chemistry
Abbreviated serial title: Anal. Chem.
Volume: v 78
Issue: n 11
Issue date: Jun 1 2006
Publication year: 2006
Pages: p 3528-3536
Language: English
ISSN: 0003-2700
CODEN: ANCHAM
Document type: Journal article (JA)
Publisher: American Chemical Society, Columbus, OH 43210-3337, United States
Abstract: Efficient sample preparation tools are the key to measuring molecular signals in a complex biological system. A novel continuous-flow isoelectric point (pI)-based sorting technique has been developed for proteins and peptides in a microfluidic chip format. It can sort biomolecules at a relatively high flow rate of up to 10 ?L/min and does not require carrier ampholytes, which can create molecular backgrounds for subsequent analysis. Furthermore, the electrophoretic field required to run the pI-based sorting is generated by the diffusion of buffer ions in situ, at the liquid junction between two laminar flows within the microfluidic channel. Utilizing the diffusion potential in combination with a pH difference between the buffers, we demonstrated a separation of binary mixtures of pI markers and proteins without applying any external field. The sorting resolution and its efficiency are sufficiently high for sample preparation and could be further improved by optimizing buffers or with an additional transverse electric field. Once fully developed, it can potentially be a pI-based sample fractionation tool for proteomic analysis of complex biomolecule samples. ? 2006 American Chemical Society.
Number of references: 21
Ei main heading: Proteins
Ei controlled terms: Polypeptides - Fluidics - pH - Electric fields - Binary mixtures - Diffusion
Uncontrolled terms: Electrophoretic field - Microfluidic chip - Complex biomolecule - Carrier ampholytes
Ei classification codes: 804.1 Organic Compounds - 815.1.1 Organic Polymers - 632.1 Hydraulics - 732.1 Control Equipment - 801.1 Chemistry, General - 701.1 Electricity: Basic Concepts & Phenomena
Treatment: Theoretical (THR); Experimental (EXP)
DOI: 10.1021/ac052156t
Database: Compendex
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