Chitosan/gelatin composite microcarrier for hepatocyte culture

Chitosan/gelatin composite microcarrier for hepatocyte culture
June 2004
Keguo Li1, Yun Wang1, Zhenchuan Miao1, Dayong Xu1, Yuefeng Tang1 and Meifu Feng1
Biotechnology Letters
Issue: Volume 26, Number 11
SpringerLink
(1) State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, P.R. China
Abstract Solid and porous chitosan/gelatin (CG) composite microcarriers were prepared by a water-in-oil emulsion process with additional freezing and lyophilization. Adult rat hepatocytes (106 cells ml?1) attached on CG microcarriers maintained at least 15 d of viability and differentiated functions. Over 15 d, unimmobilized hepatocytes released 1.34-fold less lactate dehydrogenase (LDH), and retained 1.63-, 1.51- and 1.28-fold higher albumin secretion, urea synthesis and 7-ethoxycoumarin deethylation activities, respectively, than those on collagen-coated microcarriers. The CG matrix is therefore a promising microcarrier for hepatocyte culture.
chitosan - gelatin - hepatocytes - microcarrier
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