Characterization of lignin using multi-angle laser light scattering and atomic force microscopy

Characterization of lignin using multi-angle laser light scattering and atomic force microscopy
Engineering Village 2
2006 Elsevier Inc.
Accession number: 05529619810

Title: Characterization of lignin using multi-angle laser light scattering and atomic force microscopy

Authors: Gidh, Aarti V.; Decker, Stephen R.; See, Chun H.; Himmel, Michael E.; Williford, Clint W.

Author affiliation: National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, United States

Serial title: Analytica Chimica Acta

Abbreviated serial title: Anal. Chim. Acta

Volume: v 555

Issue: n 2

Issue date: Jan 12 2006

Publication year: 2006

Pages: p 250-258

Language: English

ISSN: 0003-2670


Document type: Journal article (JA)

Publisher: Elsevier, Amsterdam, 1000 AE, Netherlands

Abstract: Small differences in the isolation techniques of lignin can result in significant changes in its molecular structure and configuration. Light scattering (evaluated at 18 different angles in a plane), Atomic Force Microscopy (AFM) and Near Infrared Spectroscopy (NIR) proved very effective for evaluating the characteristics of lignin. Zimm plots were generated using Zimm, Debye and Berry formalisms to evaluate the weight average molecular weight (MW), radius of gyration (rg), hydrodynamic radius (rh) and second virial coefficient (A2). Two types of lignin and nine different solvents were used for the study, to analyze the conformation of lignin molecules in different solvents expected to be used in lignin degradation and subsequent analysis. Absolute MW and rg decreased and the dn/dc increased when the solvent used for lignin was changed from water to sodium hydroxide. The two types of lignin also exhibited different values for all the above estimated parameters. This study also highlighted the differences between the unlyophilized and lyophilized lignin in terms of aggregation, pH dependence and stability over time. This aggregation has never been seen on a ultraviolet (UV) or refractive index (RI) detector that has been used so far for liquid chromatography (LC) reducing the reliability of lignin depolymerization data obtained without light scattering. ? 2005 Elsevier B.V. All rights reserved.

Number of references: 29

Ei main heading: Proteins

Ei controlled terms: Characterization - Light scattering - Atomic force microscopy - Microstructure - Molecular weight - Solvents - Refractive index - Agglomeration - Depolymerization

Uncontrolled terms: Molecular conformation - Multi-angle laser light scattering - Virial coefficient - Near Infrared Spectroscopy (NIR)

Ei classification codes: 804.1 Organic Compounds - 741.1 Light/Optics - 741.3 Optical Devices & Systems - 531.2 Metallography - 931.3 Atomic & Molecular Physics - 803 Chemical Agents and Basic Industrial Chemicals - 802.3 Chemical Operations - 815.2 Polymerization

Treatment: Theoretical (THR); Experimental (EXP)

DOI: 10.1016/j.aca.2005.09.023

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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