Capillary electrophoresis-sodium dodecyl sulfate of a heavily glycosylated protein: Feasibility of stability monitoring - Analytical Methods & Tools for Lyophilization

Capillary electrophoresis-sodium dodecyl sulfate of a heavily glycosylated protein: Feasibility of stability monitoring
Engineering Village 2
? 2006 Elsevier Inc
Title: Capillary electrophoresis-sodium dodecyl sulfate of a heavily glycosylated protein: Feasibility of stability monitoring

Authors: Zhang, Le; Yeung, Bernice; Liu, Jennifer

Author affiliation: Dept. Proc. Analyt. Sci. Operations, Amgen Inc., Thousand Oaks, CA 91320, United States

Serial title: American Laboratory

Abbreviated serial title: Am Lab

Volume: v 38

Issue: n 1

Issue date: January 2006

Publication year: 2006

Pages: p 34-37+10

Language: English

ISSN: 0044-7749

CODEN: ALBYBL

Document type: Journal article (JA)

Publisher: International Scientific Communications Inc., Shelton, CT 06484-0870, United States

Abstract: Traditional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is widely used in biotechnology manufacturing to monitor the purity of therapeutic proteins during formulation and process development. Recently, however, in order to establish a more robust methodology, there has been growing interest in using capillary electrophoresis-SDS to replace traditional SDS-PAGE in the therapeutic protein area.

Number of references: 10

Ei main heading: Proteins

Ei controlled terms: Glycols - Sodium compounds - Electrophoresis - Capillarity - Robustness (control systems) - Biotechnology

Uncontrolled terms: Glycosylated protein - Sodium dodecyl sulfate - Capillary electrophoresis

Ei classification codes: 804.1 Organic Compounds - 804.2 Inorganic Compounds - 701.1 Electricity: Basic Concepts & Phenomena - 801.3 Colloid Chemistry - 801.4.1 Electrochemistry - 631.1 Fluid Flow, General - 461.8 Biotechnology

Treatment: Theoretical (THR)

Database: Compendex

Compilation and indexing terms, ? 2006 Elsevier Inc. All rights reserved
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