Bacterial ghosts as antigen delivery vehicles
Bacterial ghosts as antigen delivery vehicles
Received 31 January 2004; accepted 25 January 2005. Available online 20 April 2005.
Ulrike Beate Mayra, Petra Walchera, Chakameh Azimpoura, Eva Riedmanna, b, Christoph Hallera and Werner Lubitza, b,
Advanced Drug Delivery Reviews
ScienceDirect
Advanced Drug Delivery Reviews
Abstract
The bacterial ghost system is a novel vaccine delivery system unusual in that it combines excellent natural intrinsic adjuvant properties with versatile carrier functions for foreign antigens. The efficient tropism of bacterial ghosts (BG) for antigen presenting cells promotes the generation of both cellular and humoral responses to heterologous antigens and carrier envelope structures. The simplicity of both BG production and packaging of (multiple) target antigens makes them particularly suitable for use as combination vaccines. Further advantages of BG vaccines include a long shelf-life without the need of cold-chain storage due to their freeze-dried status, they are safe as they do not involve host DNA or live organisms, they exhibit improved potency with regard to target antigens compared to conventional approaches, they are versatile with regards to DNA or protein antigen choice and size, and as a delivery system they offer high bioavailability.
Keywords: Target antigen; Gram-negative bacterial envelope; Particle presentation technology; DNA vaccine; Adjuvant; Delivery system; Bacterial ghosts
Abbreviations: APC, antigen presenting cells; APP, Actinobacillus pleuropneumoniae; BG, bacterial ghosts; CPS, cytoplasmic space; DC, dendritic cells; GFP, green fluorescent protein; IM, inner membrane; LPS, lipopolysaccharide; MBP, maltose binding protein; OM, outer membrane; OMP, outer membrane protein; PBMC, peripheral blood derived monocytic cells; PPS, periplasmic space; StrpA, streptavidin; TA, target antigen; TCP, toxin-co-regulated pili; VCG, Vibrio cholerae ghosts
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