Analytical Ultracentrifugation for Characterizing Nanocrystals and Their Bioconjugates
Analytical Ultracentrifugation for Characterizing Nanocrystals and Their Bioconjugates
Received December 14, 2004
Web Release Date: April 6, 2005
Michelle Calabretta, Jennifer A. Jamison, Joshua C. Falkner, Yunping Liu, Benjamin D. Yuhas, Kathleen S. Matthews, and Vicki L. Colvin*
Nano Lett., 5
ACS Publications
Copyright ? 2005 American Chemical Society
The Department of Biochemistry and Cell Biology and The Department of Chemistry, Rice University, Houston, Texas 77005
Abstract:
Analytical ultracentrifugation (AU) provides a general way to probe the polydispersity of nanoparticles and the formation of bioconjugates in solution. Unconjugated gold nanocrystals show sedimentation coefficient distributions that are in agreement with size distributions as measured by TEM. AU is sensitive to the size/shape changes elicited by conjugation, in this case to lactose repressor (LacI). AU data reveal saturating protein concentrations for conjugates that correspond to the measured stoichiometry of the complex under these conditions.
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